esearch Building, Rm 310
4500 San Pablo Road
Jacksonville, FL 32224
(904) 953-6372
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Bosch,
Juergen
Sent: Friday, July 13, 2012 8:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] harvesting in cold room (was: cryo for h
e, FL 32224
(904) 953-6372
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Bosch,
Juergen
Sent: Friday, July 13, 2012 8:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] harvesting in cold room (was: cryo for high salt crystal)
This is a very interesting topic I ha
Thanks, these are great links!
Evette
On Jul 13, 2012, at 7:39 PM, "Andrew Purkiss-Trew"
wrote:
> Quoting Jacob Keller :
>
>>>
>>> The expansion ratio of liquid to gaseous nitrogen is approximately 1:700,
>>> that is, 1 liter of liquid becomes 700 liters of gas (at room temperature).
>>> When
arvesting
crystals.
Mark
-Original Message-
From: Jacob Keller
mailto:j-kell...@fsm.northwestern.edu>>
To: mjvdwoerd mailto:mjvdwo...@netscape.net>>
Cc: CCP4BB mailto:CCP4BB@jiscmail.ac.uk>>
Sent: Fri, Jul 13, 2012 5:10 pm
Subject: Re: [ccp4bb] harvesting in cold room (was
in cold room (was: cryo for high salt crystal)
The expansion ratio of liquid to gaseous nitrogen is approximately 1:700, that
is, 1 liter of liquid becomes 700 liters of gas (at room temperature). When you
are in a room that is 3 (~10ft) meters tall, 6 (~18ft) meters wide and 10
(~30ft
Quoting Jacob Keller :
The expansion ratio of liquid to gaseous nitrogen is approximately 1:700,
that is, 1 liter of liquid becomes 700 liters of gas (at room temperature).
When you are in a room that is 3 (~10ft) meters tall, 6 (~18ft) meters wide
and 10 (~30ft) meters long and you assume that
-Original Message-----
> From: Radisky, Evette S., Ph.D., Ph.D.
> To: CCP4BB
> Sent: Fri, Jul 13, 2012 3:19 pm
> Subject: Re: [ccp4bb] harvesting in cold room (was: cryo for high salt
> crystal)
>
> Several have mentioned harvesting in the cold room to reduce
&g
rence materials about these things.
-Original Message-
From: Radisky, Evette S., Ph.D., Ph.D.
To: CCP4BB
Sent: Fri, Jul 13, 2012 3:19 pm
Subject: Re: [ccp4bb] harvesting in cold room (was: cryo for high salt crystal)
Several have mentioned harvesting in the cold room to reduce evaporatio
isky,
> Evette S., Ph.D. [radisky.eve...@mayo.edu]
> Sent: Saturday, July 14, 2012 7:19 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] harvesting in cold room (was: cryo for high salt
> crystal)
>
> Several have mentioned harvesting in the cold room to reduce evaporation.
Thanks much. It helps a lot.
On Jul 13, 2012, at 5:31 PM, "Henry Bellamy" wrote:
> liquid N2 expands about 600 fold to RT gas. The minimum O2 concentration is
> 19% (per OSHA I think) so if the amount of vaporized N2 is greater than 2%
> of the cold room volume you could have a problem.
On Fri, Jul 13, 2012 at 2:19 PM, Radisky, Evette S., Ph.D.
wrote:
> Several have mentioned harvesting in the cold room to reduce evaporation. I
> used to do this also as a postdoc, but I worried whether I risked nitrogen
> gas poisoning from liquid N2 boil-off, since the cold room did not seem ve
You probably already know this, but nitrogen is not at all poisonous--about
78% of the air is nitrogen. I guess you were probably worried about
asphyxiation?
JPK
On Fri, Jul 13, 2012 at 4:19 PM, Radisky, Evette S., Ph.D. <
radisky.eve...@mayo.edu> wrote:
> Several have mentioned harvesting
Several have mentioned harvesting in the cold room to reduce
evaporation. I used to do this also as a postdoc, but I worried whether
I risked nitrogen gas poisoning from liquid N2 boil-off, since the cold
room did not seem very well-ventilated. I've also hesitated to
recommend it to trainees in m
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