On Fri, Jul 13, 2012 at 2:19 PM, Radisky, Evette S., Ph.D. <radisky.eve...@mayo.edu> wrote: > Several have mentioned harvesting in the cold room to reduce evaporation. I > used to do this also as a postdoc, but I worried whether I risked nitrogen > gas poisoning from liquid N2 boil-off, since the cold room did not seem very > well-ventilated. I’ve also hesitated to recommend it to trainees in my > current lab for the same reason. Does anyone have solid information on > this? I would like to be convinced that such fears are unfounded …
Aside from safety concerns, won't this reduce the solubility? I hated harvesting high-salt conditions in the cold room for exactly this reason. -Nat
