Re: [ccp4bb] detect dsDNA

2011-10-02 Thread Dima Klenchin
>> There exists a less toxic chemical than EtBr to stain DNA: SYBR safe >> DNA stain (a fluorescence dye sold by a certain vendor). > > SYBR Safe is about 10X less sensitive though. Can you do the toothbrush test with SYBR Safe? I wouldn't do that. As it is considerably more hydrophobic, I'd ex

Re: [ccp4bb] detect dsDNA

2011-10-02 Thread Tommi Kajander
i wouldn't recoommend that. here is the info from somebody forwarded from our genetics department with regards to safety of that while back "Sybr is just as toxic/poisonous/harmful as ethidium bromide, only far more expensive. There have been very few tests concerning it's use up unti

Re: [ccp4bb] detect dsDNA

2011-10-02 Thread Jacob Keller
>> There exists a less toxic chemical than EtBr to stain DNA: SYBR safe >> DNA stain (a fluorescence dye sold by a certain vendor). > > SYBR Safe is about 10X less sensitive though. Can you do the toothbrush test with SYBR Safe? JPK *** Jacob Pearson Kell

Re: [ccp4bb] detect dsDNA

2011-10-02 Thread Dima Klenchin
There exists a less toxic chemical than EtBr to stain DNA: SYBR safe DNA stain (a fluorescence dye sold by a certain vendor). SYBR Safe is about 10X less sensitive though. I suspect that not many chemicals in the lab are less toxic/mutagenic than EthBr. The classic Ames test shows that 5 ug of

Re: [ccp4bb] detect dsDNA

2011-10-02 Thread Florian Schmitzberger
There exists a less toxic chemical than EtBr to stain DNA: SYBR safe DNA stain (a fluorescence dye sold by a certain vendor). Another benefit is to be able to use blue light, reducing UV/VIS light exposure when handling gels. Florian On Oct 2, 2011, at 11:49 AM, Edward A. Berry wrote: Ja

Re: [ccp4bb] detect dsDNA

2011-10-02 Thread Edward A. Berry
Jacob Keller wrote: I actually looked at an EtBr MSDS a while ago, and was shocked at how benign it was. I also heard from someone that they used to feed it to Argentinian cows routinely a few years back... Wikipedia says it was used as a trypanosomacidal - It's being discontinued not because

Re: [ccp4bb] detect dsDNA

2011-10-02 Thread Poul Nissen
We chewed pills with EtBr as kids in school to see if we brushed our teeth well - red colour on the edges, bad boy Poul On 01/10/2011, at 19.12, Jacob Keller wrote: > I actually looked at an EtBr MSDS a while ago, and was shocked at how > benign it was. I also heard from someone that they us

Re: [ccp4bb] detect dsDNA

2011-10-01 Thread Jacob Keller
I actually looked at an EtBr MSDS a while ago, and was shocked at how benign it was. I also heard from someone that they used to feed it to Argentinian cows routinely a few years back... JPK On Sat, Oct 1, 2011 at 1:56 AM, James Stroud wrote: > If you can reproduce the crystals and have the mate

Re: [ccp4bb] detect dsDNA

2011-10-01 Thread Phoebe Rice
011 11:36:59 +0800 >From: CCP4 bulletin board (on behalf of zq deng >) >Subject: [ccp4bb] detect dsDNA >To: CCP4BB@JISCMAIL.AC.UK > > Hi all, > . > recently,I got a crystal of protein-DNA crystal.i > used silver stainto prove that it is a protein > crystal.

Re: [ccp4bb] detect dsDNA

2011-10-01 Thread Kay Perry
If your DNA is small enough, you can run a control dsDNA lane on the gel. dsDNA will show up on silver stain but the oligomer has to be small enough to enter the gel. Kay On Sep 30, 2011, at 10:37 PM, zq deng wrote: > Hi all, > . > recently,I got a crystal of protein-DNA crystal.i used silve

Re: [ccp4bb] detect dsDNA

2011-10-01 Thread Juha Vahokoski
Also this method might be useful if crystals tolerate the treatment. Regards, Juha Fluorescence detection of nucleic acids and proteins in multi-component crystalsActa Crystallographica Section D Volume 62, Issue 2, February

Re: [ccp4bb] detect dsDNA

2011-09-30 Thread James Stroud
If you can reproduce the crystals and have the material 1. Harvest several large crystals. 2. Make several transfers to fresh mother liquor to wash. 3. Dissolve in DNA loading dye without SDS 4. Run on a native gel (e.g. 6% polyacrylamide, 0.5XTBE, etc.). 5. Include positive control lanes for prot

Re: [ccp4bb] detect dsDNA

2011-09-30 Thread Dunten, Pete W.
etin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of zq deng [dengzq1...@gmail.com] Sent: Friday, September 30, 2011 8:36 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] detect dsDNA Hi all, . recently,I got a crystal of protein-DNA crystal.i used silver stainto prove that it is a protein crystal.

[ccp4bb] detect dsDNA

2011-09-30 Thread zq deng
Hi all, . recently,I got a crystal of protein-DNA crystal.i used silver stainto prove that it is a protein crystal.Does anyone have method to detect if there is DNA in the crystal. any suggestion will be appreciated. Regards, deng