We chewed pills with EtBr as kids in school to see if we brushed our teeth well - red colour on the edges, bad boy
Poul On 01/10/2011, at 19.12, Jacob Keller <j-kell...@fsm.northwestern.edu> wrote: > I actually looked at an EtBr MSDS a while ago, and was shocked at how > benign it was. I also heard from someone that they used to feed it to > Argentinian cows routinely a few years back... > > JPK > > On Sat, Oct 1, 2011 at 1:56 AM, James Stroud <xtald...@gmail.com> wrote: >> If you can reproduce the crystals and have the material >> >> 1. Harvest several large crystals. >> 2. Make several transfers to fresh mother liquor to wash. >> 3. Dissolve in DNA loading dye without SDS >> 4. Run on a native gel (e.g. 6% polyacrylamide, 0.5XTBE, etc.). >> 5. Include positive control lanes for protein, DNA, and complex. >> 6. Stain with ETBr. Take a picture. >> 7. Wash out the ethidium in accordance with state, local, federal, UN, laws, >> filling out the proper documentation ad nauseum. Take a safety class just to >> be sure you didn't miss something. Hug a bureaucrat. >> 8. Stain with coomassie. Take a picture. >> >> That should tell you more than you need to know. >> >> James >> >> On Sep 30, 2011, at 9:36 PM, zq deng wrote: >> >>> Hi all, >>> . >>> recently,I got a crystal of protein-DNA crystal.i used silver stainto prove >>> that it is a protein crystal.Does anyone have method to detect if there is >>> DNA in the crystal. >>> any suggestion will be appreciated. >>> >>> Regards, >>> deng >> > > > > -- > ******************************************* > Jacob Pearson Keller > Northwestern University > Medical Scientist Training Program > cel: 773.608.9185 > email: j-kell...@northwestern.edu > *******************************************
