I read somewhere (I don’t have the ref, sorry) that it is because the phases
are directly measured in cryoEM (since it is an imaging technique), and they
are often more accurate than the phases of crystallographic maps.
On this basis, if you can find a structure that has been solved both by cryo
s the
values are the same.
Best wishes,
Colin
From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>>
On Behalf Of anna anna
Sent: Thursday, September 26, 2024 11:13 AM
To: ccp4bb mailto:ccp4bb@jiscmail.ac.uk>>
Subject: [ccp4bb] contour level for cryoEM deposition: coot vs chime
Sorry, I think the answer is on Colin's reply.
Best wishes, Jon Cooper.
jon.b.coo...@protonmail.com
Sent from Proton Mail Android
Original Message
On 26/09/2024 17:56, Jon Cooper wrote:
> Another thing I am curious about is how a map can look good at 5.5 sigma
> because an X-
Another thing I am curious about is how a map can look good at 5.5 sigma
because an X-ray one probably wouldn't unless it was atomic resolution. Don't
worry if there isn't a simple explanation.
Best wishes, Jon Cooper.
jon.b.coo...@protonmail.com
Sent from Proton Mail Android
Original
board On Behalf Of anna anna
Sent: Thursday, September 26, 2024 11:13 AM
To: ccp4bb
Subject: [ccp4bb] contour level for cryoEM deposition: coot vs chimera
Dear all,
I am depositing my first cryoEM structure and I am facing the following problem
concerning the contour level of the map.
Briefly
Hello Anna
I have no idea why the map format is unacceptable without more details of how
you created the file. Presumably it is in ccp4 format, but there are others. Is
it a binary file or humanly readable? It's EM so cell dimensions are
artificial, etc, I think.
Best wishes, Jon Cooper.
jon.b
Dear all,
I am depositing my first cryoEM structure and I am facing the following
problem concerning the contour level of the map.
Briefly, where do I find the value for the contour level to associate to
the uploaded map in Coot?
Long story: I worked with coot and I am happy with my map when I se
