Dear all, I am depositing my first cryoEM structure and I am facing the following problem concerning the contour level of the map.
Briefly, where do I find the value for the contour level to associate to the uploaded map in Coot? Long story: I worked with coot and I am happy with my map when I set the contour level at 0.115 V = 5.5 rmsd (actually "happy" is not the best definition, I feel quite uncomfortable with this "by feeling" assignment but, to what I understood, this is how it works...). Upon deposition, when I upload maps, a contour level is required but the values from coot are not accepted, I have to provide the contour level relative to the map value, like the one that you set in Chimera. Thus I opened the map in Chimera and I set a contour level "by eye" but the validation report revealed some problems: the total volume of the map is too low and too many residues are highlighted as out of density. Probably I should low the contour level but I don't want to go for a trial-and-error approach, there must be a way to convert the contour level from coot (the one that I used for modelling) in the one required for deposition! Any advice? Thank you, Anna ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
