I've tried to post my reply to this quite a few times but as best I can
tell (and my friends can tell from their CCP4bb subscriptions) I have been
foiled by jiscmail every time. Hopefully this will go through. And
hopefully I'm not reposting stuff I already sent. Here we go:
Okay, the consensus he
There is an alternative method that does not rely on hydrphobic interaction
of dye with the protein interior: it relies instead on reaction between
fluorogenic dye and interior cysteine residues of the protein. When protein
melts these Cys residues become exposed, react with the dye and generate
fl
Hi Theresa,
Check if this works in your case
http://www.sciencedirect.com/science/article/pii/S0969212608000609
good luck !!
On Sat, Apr 12, 2014 at 4:38 PM, Theresa Hsu wrote:
> Dear all
>
> Does anyone has experience with Thermofluor assay to find the substrate
> transported/binding by a mem
interest. However, I have
never personally tried it with a membrane protein
.
Best,,
,
D
From: Theresa Hsu [theresah...@live.com]
Sent: 12 April 2014 09:38
To: ccp4bb
Subject: [ccp4bb] Thermofluor assay
Dear all
Does anyone has experience with
Dear all
Does anyone has experience with Thermofluor assay to find the substrate
transported/binding by a membrane protein? My protein does not have any similar
structures and the substrate suggested by sequence analysis is not being
transported in proteoliposome. I know ITC is good but I am lo
