Hi Theresa, Check if this works in your case http://www.sciencedirect.com/science/article/pii/S0969212608000609
good luck !! On Sat, Apr 12, 2014 at 4:38 PM, Theresa Hsu <theresah...@live.com> wrote: > Dear all > > Does anyone has experience with Thermofluor assay to find the substrate > transported/binding by a membrane protein? My protein does not have any > similar structures and the substrate suggested by sequence analysis is not > being transported in proteoliposome. I know ITC is good but I am looking > for a more high-throughput way. > > Thank you. > -- rashmi