Re: [ccp4bb] TEV vs HRV3C

2022-12-09 Thread Artem Evdokimov
f tags sequentially as needed by > your purification strategies. > > HTH, > > Dave > > *Dr David C. Briggs CSci MRSB* > Principal Laboratory Research Scientist > Signalling and Structural Biology Lab > The Francis Crick Institute > London, UK > == > about.me/d

Re: [ccp4bb] TEV vs HRV3C

2022-12-08 Thread Dom Bellini - MRC LMB
bulletin board on behalf of Gloria Borgstahl *Sent:* Wednesday, 7 December 2022, 20:26 *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* [ccp4bb] TEV vs HRV3C *External Sender:* Use caution. Hello my fellow structural biologists,  I am contemplating why some choose the HRV3C protease site over TEV for their

Re: [ccp4bb] TEV vs HRV3C

2022-12-07 Thread Nikolay Dobrev
==http://about.me/david_briggs > > > > > > > > > - > > > From: CCP4 bulletin board > > mailto:CCP4BB@JISCMAIL.AC.UK > on behalf of Gloria Borgstahl > > > mailto:gborgst...@gm

Re: [ccp4bb] TEV vs HRV3C

2022-12-07 Thread Lior Almagor
> about.me/david_briggs <http://about.me/david_briggs> >> >> From: CCP4 bulletin board > <mailto:CCP4BB@JISCMAIL.AC.UK>> on behalf of Gloria Borgstahl >> mailto:gborgst...@gmail.com>> >> Sent: Wednesday, 7 December 2022, 20:26 >> To: C

Re: [ccp4bb] TEV vs HRV3C

2022-12-07 Thread Lau Kelvin
CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: [ccp4bb] TEV vs HRV3C External Sender: Use caution. Hello my fellow structural biologists, I am contemplating why some choose the HRV3C protease site over TEV for their fusion proteins. Does any

Re: [ccp4bb] TEV vs HRV3C

2022-12-07 Thread Chun Luo
: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] TEV vs HRV3C Hello my fellow structural biologists, I am contemplating why some choose the HRV3C protease site over TEV for their fusion proteins. Does anyone know? Can HRV3C be made easily in homelab? Does anyone have a plasmid? Thank you, G

Re: [ccp4bb] TEV vs HRV3C

2022-12-07 Thread Cyprian Cukier
@JISCMAIL.AC.UK Subject: Re: [ccp4bb] TEV vs HRV3C We use the HRV3C protease site frequently in our lab. It provides better on-bead/column cleavage than TEV. We have a plasmid as we make 3C protease in our lab and keep stocks in our -80C. I can send you our protocol if you wish. Crissy Crissy L

Re: [ccp4bb] TEV vs HRV3C

2022-12-07 Thread Crissy L Tarver
. From: CCP4 bulletin board on behalf of Gloria Borgstahl Sent: Wednesday, December 7, 2022 12:26 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] TEV vs HRV3C Hello my fellow structural biologists, I am contemplating why some choose the HRV3C protease site over TEV for

Re: [ccp4bb] TEV vs HRV3C

2022-12-07 Thread David Briggs
n, UK == about.me/david_briggs<http://about.me/david_briggs> From: CCP4 bulletin board on behalf of Gloria Borgstahl Sent: Wednesday, 7 December 2022, 20:26 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] TEV vs HRV3C External Sender: Use caution. Hell

[ccp4bb] TEV vs HRV3C

2022-12-07 Thread Gloria Borgstahl
Hello my fellow structural biologists, I am contemplating why some choose the HRV3C protease site over TEV for their fusion proteins. Does anyone know? Can HRV3C be made easily in homelab? Does anyone have a plasmid? Thank you, G