HRV3C cut of an N-terminal tag usually leaves GP at the N-terminus. TEV usually leaves G/S. Structural biologists may prefer TEV. However, HRV3C has more robust activity than TEV. HRV3C is a better choice for removal of C-terminal tags.
To save precious time, Accleagen has Turbo3C (http://www.accelagen.com/Turbo3C.htm) available at a reasonable price. It has dual GST- and His-tags for easy removal. We have other enzymes for protein purification. Chun Luo Accelagen From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Gloria Borgstahl Sent: Wednesday, December 7, 2022 12:26 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] TEV vs HRV3C Hello my fellow structural biologists, I am contemplating why some choose the HRV3C protease site over TEV for their fusion proteins. Does anyone know? Can HRV3C be made easily in homelab? Does anyone have a plasmid? Thank you, G _____ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> &A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
