Subject: [ccp4bb] Superdex 200 PC columns
Sorry for perhaps off-topic question but I am writing to ask if anyone has
experience in using Amersham Superdex 200 PC 3.2/30 columns in conjuction
with
the Precision Column holder on a standard (not SMART system) AKTA FPLC.
At the
moment using a Super
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Hi,
You're right, there are two cell path sizes in AKTA systems: 2 mm and 10
mm. I don't know what path had the SMART. You definitely need the second
one if you want to work with low protein concentrations.
Miguel
En/na Juergen Bosch ha escrit:
> H
Hi all,
Isn't the flow cell volume the limiting factor for your detection limit
? I believe for the "old" Aktas (~5-8 years old) there were two cell
sizes a laerger one and a smaller one, and I don't think it is the same
size as in the SMART system. But I might be wrong.
Jürgen
[EMAIL PROTE
Hi Robert,
In my experience, there is no problem running a SMART column on an AKTA
FPLC or Explorer. I have been using the same S75, S200, and Superose6
columns on all three systems depending on which one is available that day.
I typically load 50 ul sample, between 10-0.01 mg/ml. The peak width I
It greatly depends on the state of your instrument - how well you took
care of the optical elements, etc. It also depends on the initial
concentration of the sample (and therefore the peak volume at detection
time) and on the optical activity of the sample and the buffer. If the
stars align and eve
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Hi Robert,
I have run those PC 2.3/30 with ~10 ug of protein (of course this
depends on the protein) and still get a useful signal at 280 nm. If you
don't have anything interfering at ~215 nm and your HPLC system can read
at that wavelength, you can e
Sorry for perhaps off-topic question but I am writing to ask if anyone has
experience in using Amersham Superdex 200 PC 3.2/30 columns in conjuction with
the Precision Column holder on a standard (not SMART system) AKTA FPLC. At the
moment using a Superdex 200 10/300 column on a standard AKTA I ca
