Sorry for perhaps off-topic question but I am writing to ask if anyone has
experience in using Amersham Superdex 200 PC 3.2/30 columns in conjuction with
the Precision Column holder on a standard (not SMART system) AKTA FPLC. At the
moment using a Superdex 200 10/300 column on a standard AKTA I can get a
reasonable signal from down to about 20 ug protein - I am wondering how much
less protein I will be able to use with the narrower Superdex 200 PC 3.2/30
column when I hook it up to the same FPLC via the Precision Column holder.
