Hi Robert, In my experience, there is no problem running a SMART column on an AKTA FPLC or Explorer. I have been using the same S75, S200, and Superose6 columns on all three systems depending on which one is available that day. I typically load 50 ul sample, between 10-0.01 mg/ml. The peak width I get is ~150ul, with 70-80% of the original concentration at the height of the peak. On the FPLC/Explorer I replace the standard tubing to shorter and more narrow tubing and bypass the conductivity meter to reduce the amount of dead volume. Finally, for the very low protein concentrations, you're better of using the Explorer, which allows you to measure the absorption at 220-230nm.
Meindert > Sorry for perhaps off-topic question but I am writing to ask if anyone has > experience in using Amersham Superdex 200 PC 3.2/30 columns in conjuction > with > the Precision Column holder on a standard (not SMART system) AKTA FPLC. > At the > moment using a Superdex 200 10/300 column on a standard AKTA I can get a > reasonable signal from down to about 20 ug protein - I am wondering how > much > less protein I will be able to use with the narrower Superdex 200 PC > 3.2/30 > column when I hook it up to the same FPLC via the Precision Column holder. >
