Hi Gloria,
I have extensively used SUMO and echoing Artem's sentiment. SUMO has worked
well in my hands for most instances. The additional benefit is that,
assuming you use His-SUMO-target, following His-tag affinity purification,
you can cleave the SUMO tag, followed by reverse IMAC (i.e., collec
Hi Gloria!
What’s the point of publishing a nice paper if nobody reads it? *Sigh* (see
reference below, we’re happy to share vectors).
So yes, SUMO fusions have definitely worked for us; this strategy is our first
choice for expressing any new soluble protein, and we use it for a lot of
membra
Affirmative on all these questions:
SUMO works well
SUMO protease is super easy to make in house
As to which one - regular SUMO for E. coli and SUMO* for mammalian or
insect cell systems because regular SUMO may get cleaved by the intrinsic
protease. SUMO* is a triple mutant, with corresponding m
Hello friends, Yet another kinda off topic question from me. I am looking
into SUMO tags for my protein of interest to help solubility. Has this
worked for you? If so which SUMO and protease do you use? Can the
protease be made in house? So far I am looking at the pET SUMO system. Is
this th
