Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit

2013-04-30 Thread sonali dhindwal
: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, 30 April 2013 1:42 AM Subject: Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit and 2VAK...average Bs for the twelve, sequence identical, chains vary from 28 to 53. On 29 Apr 2013, at 22:09, David Schuller wrote: On 04/29/13 12

Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit

2013-04-29 Thread Mark van Raaij
and 2VAK...average Bs for the twelve, sequence identical, chains vary from 28 to 53. On 29 Apr 2013, at 22:09, David Schuller wrote: > On 04/29/13 12:26, Roger Rowlett wrote: >> FYI, I do know of one example of a solved structure where some of the >> molecules in the ASU are poorly defined. In

Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit

2013-04-29 Thread David Schuller
On 04/29/13 12:26, Roger Rowlett wrote: FYI, I do know of one example of a solved structure where some of the molecules in the ASU are poorly defined. In 1EKJ, 4 of the 8 molecules in the ASU have low B-factors (mid 30s) and 4 have high B-factors (50s-60s). In the unit cell these layers alterna

Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit

2013-04-29 Thread sonali dhindwal
: sonali dhindwal Sent: Monday, 29 April 2013 10:03 PM Subject: Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit Where are the phases coming from? MR? F On Apr 28, 2013, at 10:02 PM, sonali dhindwal wrote: > Dear All, > > We are working on a crystal

Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit

2013-04-29 Thread Fischmann, Thierry
ve forever.” From: Eleanor Dodson <mailto:eleanor.dod...@york.ac.uk> To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Sent: Monday, 29 April 2013 4:36 PM Subject: Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit There isnt much informat

Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit

2013-04-29 Thread Roger Rowlett
ever.” *From:* Eleanor Dodson *To:* CCP4BB@JISCMAIL.AC.UK *Sent:* Monday, 29 April 2013 4:36 PM *Subject:* Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit There isnt much information here! Funny that 3 chains are poor - does that mean one and a

Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit

2013-04-29 Thread sonali dhindwal
: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit There isnt much information here! Funny that 3 chains are poor - does that mean one and a half heterodimers? I presume you have checked spacegroup? Zanuda will test to see if any higher symmetry is present.. Eleanor

Re: [ccp4bb] Poor electron density in some of the chains in an asymmetric unit

2013-04-29 Thread Eleanor Dodson
There isnt much information here! Funny that 3 chains are poor - does that mean one and a half heterodimers? I presume you have checked spacegroup? Zanuda will test to see if any higher symmetry is present.. Eleanor On 29 April 2013 06:02, sonali dhindwal wrote: > Dear All, > > We are working on

[ccp4bb] Poor electron density in some of the chains in an asymmetric unit

2013-04-28 Thread sonali dhindwal
Dear All, We are working on a crystal structure with 12 molecules in an asymmetric unit. It is a heteromer and each chain is made up of two type of chains. Therefore there are 24 chains in an  asymmetric unit. It is giving solution in P1. Rfactor and Rfree has reached 20.25 and 25.6 at a res