Dear Eleanor,
Thanks for the suggestion, I just checked on the Zanuda program, it is also
giving P1 as the best possible spacegroup for the molecule.
and by not refining well, I meant for the electron density which is broken at
many places at main chain, and poor electron density for the side chains which
are otherwise good in other chains.
Regards
--
Sonali Dhindwal
“Live as if you were to die tomorrow. Learn as if you were to live forever.”
________________________________
From: Eleanor Dodson <eleanor.dod...@york.ac.uk>
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, 29 April 2013 4:36 PM
Subject: Re: [ccp4bb] Poor electron density in some of the chains in an
asymmetric unit
There isnt much information here!
Funny that 3 chains are poor - does that mean one and a half heterodimers?
I presume you have checked spacegroup? Zanuda will test to see if any higher
symmetry is present..
Eleanor
On 29 April 2013 06:02, sonali dhindwal <sonali11dhind...@yahoo.co.in> wrote:
Dear All,
>
>
>We are working on a crystal structure with 12 molecules in an asymmetric unit.
>It is a heteromer and each chain is made up of two type of chains. Therefore
>there are 24 chains in an asymmetric unit.
>
>
>
>It is giving solution in P1. Rfactor and Rfree has reached 20.25 and 25.6 at a
>resolution of 1.8Angstrom
>
>
>
>All the chains are refining well and have good electron density except 3
>chains which have very poor electron density in most of the region.
>
>
>
>Whereas, the crystal structure of variants of the same protein we have solved
>earlier refined well and has very good electron density in all the chains.
>
>
>What could be the possible reason for these three chains which are not
>refining properly. Any suggestions will be very useful.
>
>
>Thanks and Regards
>
>
>--
>Sonali Dhindwal
>
>“Live as if you were to die tomorrow. Learn as if you were to live forever.”
