On 04/29/13 12:26, Roger Rowlett wrote:
FYI, I do know of one example of a solved structure where some of the
molecules in the ASU are poorly defined. In 1EKJ, 4 of the 8 molecules
in the ASU have low B-factors (mid 30s) and 4 have high B-factors
(50s-60s). In the unit cell these layers alternate. It is possible, if
everything else has been excluded, that your crystal has a similar
crystal-packing issue.
Another is 3HDH.
Pig short chain L-3-hydroxyacyl-CoA dehydrongenase revisited: sequence
analysis and crystal structure determination.
Barycki, et al (1999) Protein Science 8: 2010-2018.
"Examination of the map in the region of subunit C revealed that
the electron density was considerably weaker for the third subunit
compared to the other subunits..."
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu
