In addition to all the excellent suggestions to perform seeding, you could also
try sitting drop vapor diffusion, and also different temperatures. Sometimes
moving from 20 to 4 degrees does the trick.
Diana
**
Diana R. Tomchick
Professor
Departmen
Hi Nikolaus
I completely agree with Claude's comments. Microseeding is the first thing
to try. You would like to find conditions where you *don't *get crystals
without seeding, but you *do *get crystals with seeding. Then, just by
diluting the seed stock, you can control nucleation. Look at D'
Le 09/09/2019 à 17:22, Nikolas a
écrit :
Dear
crystalgrowers,
I am
currently working with a protein that appeared to be
friendly but turned out it was not the case.
I found
Hi Nikolas
The first thing I would try is to solubilise the protein a bit more, maybe by
adding a few % (1-5%) glycerol or ethylene glycol to the growth conditions when
you set up the drops.
The next thing would be to experiment with ratio (protein:precipitant) and drop
size.
Lastly, trial ad
Dear crystalgrowers,
I am currently working with a protein that appeared to be friendly but
turned out it was not the case.
I found myself to face -in the scale up- the opposite of the usual problem
of nucleation (I really love how this topic finds new ways to make fun of
me). In 24-well plates, h
