In addition to all the excellent suggestions to perform seeding, you could also try sitting drop vapor diffusion, and also different temperatures. Sometimes moving from 20 to 4 degrees does the trick.
Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry UT Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu<mailto:diana.tomch...@utsouthwestern.edu> (214) 645-6383 (phone) (214) 645-6353 (fax) On Sep 9, 2019, at 10:22 AM, Nikolas <nikolas.ca...@gmail.com<mailto:nikolas.ca...@gmail.com>> wrote: Dear crystalgrowers, I am currently working with a protein that appeared to be friendly but turned out it was not the case. I found myself to face -in the scale up- the opposite of the usual problem of nucleation (I really love how this topic finds new ways to make fun of me). In 24-well plates, hanging-drop, for the same condition but in different drops I found few big but intergrown crystals and/or a full with microcrystals. Sometimes also in the same well, when having more drops. I already decreased the concentration to less than 4mg/mL, made small adjustments in the optimizations - both with apo and ligand samples, used Al's oil. I have read about the "containerless crystallization" but since I cannot obtain the sample myself I would like to know if there are any experiences and/or if there are suggestions for solving this problem. Many thanks! Best regards, Nikolas ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ UT Southwestern Medical Center The future of medicine, today. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
