Dear crystalgrowers, I am currently working with a protein that appeared to be friendly but turned out it was not the case. I found myself to face -in the scale up- the opposite of the usual problem of nucleation (I really love how this topic finds new ways to make fun of me). In 24-well plates, hanging-drop, for the same condition but in different drops I found few big but intergrown crystals and/or a full with microcrystals. Sometimes also in the same well, when having more drops. I already decreased the concentration to less than 4mg/mL, made small adjustments in the optimizations - both with apo and ligand samples, used Al's oil.
I have read about the "containerless crystallization" but since I cannot obtain the sample myself I would like to know if there are any experiences and/or if there are suggestions for solving this problem. Many thanks! Best regards, Nikolas ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
