Hi Liuqing,
As Eleanor suggested, if your structure is expected to contain alpha
helices, you might
want to give ARCIMBOLDO_LITE a try, as your resolution is still within the
scope of the method.
In case you have any suspicion of the fold that might be and it contains
beta sheets,
you can try ARCI
Hi,
it seems you haven't found a solution. If there is no model you might
want to try experimental phasing. With that resolution and the right sg
a S-SAD might be possible. So you might not even need a HA derivative.
Cheers
Christian
Am 26.10.2016 um 14:53 schrieb SUBSCRIBE CCP4BB Liuqing
Can you push the resolution higher?
Is there any anomalous signal?
Maybe you could try Archimoldo to look for helical fragments?
BUT these approaches work better the higher the resolution data...
Speaking as one who has failed many times with sort of problem!
Good luck Eleanor
On 26 October 2016
Hello!
I have a crystal diffracted to 2Å, and have no homology structure. As, i can't
repeat the crystallization, so i try using Balbe and MorDa program, the MorDa
give a partial model with Q socre 0.46, Rwork/Rfree 0.50/0.51. it's hard to
rebuilt using coot, and when i use Buccaner or Arp/w
