orks for your situation.
That unfortunately is about as good as it gets for rules.
br
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Joe
Sent: Friday, May 01, 2009 7:23 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Hanging vs. Sitting
Sure, t
Sure, there are differences between these two methods, but no systematic
study has been reported showing one is better than the other in terms of
getting initial hits. Since we have a crystallization robot, I routinely
set up sitting drops for initial screens and hanging drops (manually)
for op
Sorry, disagree again: with the right plate type (e.g. SwissCi plates),
it's far far easier from sitting drop, because:
1. you don't have to muck around with flipping over the cover slip,
instead just cut the seal
2. you have more time, because your drop does not evaporate as quickly
(see earli
It's also easier to fish the crystals out of the solution with a
hanging drop.
Simon
On 1 May 2009, at 06:35, Debajyoti Dutta wrote:
Hi,
>From the experiance of mine I can tell you that the crystal size
sometimes matters between these two methods. Hanging drop may yield
bigger crysta
Hi,
>From the experiance of mine I can tell you that the crystal size sometimes
>matters between these two methods. Hanging drop may yield bigger crystals than
>sitting drop, that may be due to the evaporation rate(surface area). Hanging
>drop allow us to set different protocols also like f
Hi, I beg to differ. Evaporation rates change dramatically between a
drop lying on a flat surface open to air flow; a drop lying in a
depression; and a drop lying in a depression with some reservoir
solution next to it. We're talking seconds to minutes.
So especially when using robotics to
There is really not much difference in terms of setup between hanging and
sitting drop, especially if the drops are set up on tape. Visualization is
also usually easier with hanging drops.
Bostjan
On 1/05/09 1:37 AM, "Poul Nissen" wrote:
> We often find results to be very different between han
We often find results to be very different between hanging and sitting
drops (equilibration kinetics for one may be the explanation). Then
there's the good thing of hanging drops that crystals rarely stick to
the surface of the support facilitating the mounting procedure, in
particular for
I have noticed that a significant majority of crystallizations are done in
hanging- rather than sitting-drop configuration, and considering the
significant extra labor involved in hanging drops, can only understand this
preference as a historical bias. I understand that sometimes one technique
