Sure, there are differences between these two methods, but no systematic
study has been reported showing one is better than the other in terms of
getting initial hits. Since we have a crystallization robot, I routinely
set up sitting drops for initial screens and hanging drops (manually)
for optimization thereafter. As long as I have no problem getting
reproducible conditions, I will stick to the one I found most efficient
and convenient for myself.
Joe
Frank von Delft wrote:
Sorry, disagree again: with the right plate type (e.g. SwissCi
plates), it's far far easier from sitting drop, because:
1. you don't have to muck around with flipping over the cover slip,
instead just cut the seal
2. you have more time, because your drop does not evaporate as quickly
(see earlier mail)
3. if the crystal sticks, just poke an acupuncture needle into the
plastic below it: off it pops.
phx
Simon Kolstoe wrote:
It's also easier to fish the crystals out of the solution with a
hanging drop.
Simon
On 1 May 2009, at 06:35, Debajyoti Dutta wrote:
Hi,
>From the experiance of mine I can tell you that the crystal size
sometimes matters between these two methods. Hanging drop may yield
bigger crystals than sitting drop, that may be due to the
evaporation rate(surface area). Hanging drop allow us to set
different protocols also like free interface diffusion, area covered
by the drp etc.
These informations are gained purely by experiance.
cheers
Deb
On Thu, 30 Apr 2009 20:40:35 +0530 wrote
>I have noticed that a significant majority of crystallizations are
done in
>hanging- rather than sitting-drop configuration, and considering the
>significant extra labor involved in hanging drops, can only
understand this
>preference as a historical bias. I understand that sometimes one
technique
>works and not the other, but all things being equal, why is hanging
drop
>still "hanging around?" Any insights appreciated...
>
>Jacob Keller
>
>*******************************************
>Jacob Pearson Keller
>Northwestern University
>Medical Scientist Training Program
>Dallos Laboratory
>F. Searle 1-240
>2240 Campus Drive
>Evanston IL 60208
>lab: 847.491.2438
>cel: 773.608.9185
>email: j-kell...@northwestern.edu <mailto:j-kell...@northwestern.edu>
>*******************************************
>
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