Hi,
>From the experiance of mine I can tell you that the crystal size sometimes >matters between these two methods. Hanging drop may yield bigger crystals than >sitting drop, that may be due to the evaporation rate(surface area). Hanging >drop allow us to set different protocols also like free interface diffusion, >area covered by the drp etc. These informations are gained purely by experiance. cheers Deb On Thu, 30 Apr 2009 20:40:35 +0530 wrote >I have noticed that a significant majority of crystallizations are done in >hanging- rather than sitting-drop configuration, and considering the >significant extra labor involved in hanging drops, can only understand this >preference as a historical bias. I understand that sometimes one technique >works and not the other, but all things being equal, why is hanging drop >still "hanging around?" Any insights appreciated... > >Jacob Keller > >******************************************* >Jacob Pearson Keller >Northwestern University >Medical Scientist Training Program >Dallos Laboratory >F. Searle 1-240 >2240 Campus Drive >Evanston IL 60208 >lab: 847.491.2438 >cel: 773.608.9185 >email: j-kell...@northwestern.edu >******************************************* >