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From: Mirek Cygler
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tue, 2 November, 2010 9:16:37 PM
Subject: [ccp4bb] Glutathione sepharose
Hello,
For various reasons we are frequently expressing proteins with a GST
tag. The glutathione
I am using the glutathione sepharose 4B beads from GE. I had noticed that the
capture efficiency does decrease with usage, though I have successfully
regenerated the beads at least 20 times and I am still using them.
I regenerate using 2CV of 6M guanidine after each run and every 5 runs with 70
Hi,
Well you did not mention which company makes your GST sepharose, but I
used GE GST Sepharose 4B quite often and was able to reuse it quite a
few times.
Chris
On Tue, 2010-11-02 at 11:46 -0400, Mirek Cygler wrote:
> Hello,
> For various reasons we are frequently expressing proteins wit
Hello,
For various reasons we are frequently expressing proteins with a GST
tag. The glutathione sepharose beads that we are using for affinity
purification seem to be difficult to regenerate and we see much lower
capacity when used the second time. We are following the manufacturer's
instr
