Hello,
For various reasons we are frequently expressing proteins with a GST
tag. The glutathione sepharose beads that we are using for affinity
purification seem to be difficult to regenerate and we see much lower
capacity when used the second time. We are following the manufacturer's
instructions for regeneration but this not very effective process as opposed
to NiNTA which can regenerate multiple times. Due to this, the purification
of GST-tagged proteins is quite costly. Do you know of a GSH-sepharose that
can be successfully regenerated several times? Any comments will be greatly
appreciated.
Mirek
