Hi, Well you did not mention which company makes your GST sepharose, but I used GE GST Sepharose 4B quite often and was able to reuse it quite a few times.
Chris On Tue, 2010-11-02 at 11:46 -0400, Mirek Cygler wrote: > Hello, > For various reasons we are frequently expressing proteins with a GST > tag. The glutathione sepharose beads that we are using for affinity > purification seem to be difficult to regenerate and we see much lower > capacity when used the second time. We are following the manufacturer's > instructions for regeneration but this not very effective process as opposed > to NiNTA which can regenerate multiple times. Due to this, the purification > of GST-tagged proteins is quite costly. Do you know of a GSH-sepharose that > can be successfully regenerated several times? Any comments will be greatly > appreciated. > > Mirek -- Dr. Christopher Browning Post-Doctor to Prof. Petr Leiman EPFL BSP-416 1015 Lausanne Switzerland Tel: 0041 (0) 02 16 93 04 40
