Re: [ccp4bb] Experimental design and response surface methods for crystal optimization

2009-05-26 Thread Patrick Shaw Stewart
   US toll-free 1-877-225-2034  Regd. England 2177994, VAT Reg. GB 480 7371 36 -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Benda, Christian Sent: 15 May 2009 15:07 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Experimental design and respon

Re: [ccp4bb] Experimental design and response surface methods for crystal optimization

2009-05-15 Thread Charles W. Carter Jr.
Christian, I happened to see your post to the CCP4 bulletin board. In answer to your question, we continue to base all of our work on proper experimental design. Two papers cited below document the flexibility and power of response surface methods in biochemical experimentation, especiall

[ccp4bb] Experimental design and response surface methods for crystal optimization

2009-05-15 Thread Benda, Christian
Dear all I recently came across the "experimental design" method for systematic optimization of multiparametric problems like macromolecular crystal growth. (The response surface concept has been introduced to protein crystallization by Carter et al. (e.g. Meth. Mol. Biol, vol. 363) and should t