Hi Christian We often use a simple approach where people design their experiments using rational multivariate design, but then simply use the best well that is found as the centre of the next round of experiments. This means that you don't have to score all the wells - which is time-consuming!
There's more info at http://www.douglas.co.uk/rat_des.htm Patrick -- patr...@douglas.co.uk Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Benda, Christian Sent: 15 May 2009 15:07 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Experimental design and response surface methods for crystal optimization Dear all I recently came across the "experimental design" method for systematic optimization of multiparametric problems like macromolecular crystal growth. (The response surface concept has been introduced to protein crystallization by Carter et al. (e.g. Meth. Mol. Biol, vol. 363) and should theoretically enable optimization of a multiparatmetric problem by simultaneous variation of several variables). I am wondering if anyone is actually making use of this method on a regular basis and what their experiences are. It would also be interesting to now if tools (web-based) are available to help in designing and evaluating experiments. Any comment appreciated! Thanks very much Christian
