Re: [ccp4bb] Disappearing crystals

2011-11-29 Thread Patrick Shaw Stewart
gt; Thanks again to all you, > > Christine > > > > -Original Message- > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of > Enrico Stura > Sent: Monday, November 28, 2011 1:45 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp

Re: [ccp4bb] Disappearing crystals

2011-11-29 Thread Enrico Stura
, Christine -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Enrico Stura Sent: Monday, November 28, 2011 1:45 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Disappearing crystals When advice on crystallization is needed, it is important to give

Re: [ccp4bb] Disappearing crystals

2011-11-28 Thread Artem Evdokimov
> From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Harman, > Christine [christine.har...@fda.hhs.gov] > Sent: Monday, November 28, 2011 12:43 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Disappearing crystals > > Hi, > Thank yo

Re: [ccp4bb] Disappearing crystals

2011-11-28 Thread Mathews, Irimpan I.
] Sent: Monday, November 28, 2011 12:43 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Disappearing crystals Hi, Thank you so much for your replies. A lot of you have mentioned fluctuations in temp as the major contributor. And a few of you have asked for more details of my protein/buffer and

Re: [ccp4bb] Disappearing crystals

2011-11-28 Thread Harman, Christine
, Christine -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Enrico Stura Sent: Monday, November 28, 2011 1:45 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Disappearing crystals When advice on crystallization is needed, it is important to give

Re: [ccp4bb] Disappearing crystals

2011-11-28 Thread Fischmann, Thierry
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jan Dohnalek Sent: Monday, November 28, 2011 1:47 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Disappearing crystals We have seen such behaviour connected to temperature fluctuations. Jan On Mon, Nov 28, 2011

Re: [ccp4bb] Disappearing crystals

2011-11-28 Thread Jan Dohnalek
We have seen such behaviour connected to temperature fluctuations. Jan On Mon, Nov 28, 2011 at 6:04 PM, Harman, Christine < christine.har...@fda.hhs.gov> wrote: > Hi All, > I have just noticed a very strange thing and need some help in > understanding it. I recently found two crystals in a co

Re: [ccp4bb] Disappearing crystals

2011-11-28 Thread Enrico Stura
When advice on crystallization is needed, it is important to give details of the protein concentration, the buffer the protein is in as well as the method used to grow the crystals. Problem: The crystallization conditions are essentially low salt: 100mM buffer and only 50mM CaCl2. So the bu

Re: [ccp4bb] Disappearing crystals

2011-11-28 Thread YoungJin
On 11/28/11 12:04 PM, Harman, Christine wrote: Hi All, I have just noticed a very strange thing and need some help in understanding it. I recently found two crystals in a condition from a screen (0.05M Calcium chloride dihydrate, 0.1M M Bis-Tris pH6.5 and 30% PEG MME 550). The small crystals

Re: [ccp4bb] Disappearing crystals

2011-11-28 Thread David Schuller
On 11/28/11 12:04, Harman, Christine wrote: Hi All, I have just noticed a very strange thing and need some help in understanding it. I recently found two crystals in a condition from a screen (0.05M Calcium chloride dihydrate, 0.1M M Bis-Tris pH6.5 and 30% PEG MME 550). The small crystals ap