Yes, and if they don't reappear it may be worth moving the trail to an incubator set at a slightly different temperature than "room temperature". You may have to try both lower or higher temperatures if you have no idea how the temperature may have changed (it's the temperature of the room when the crystals showed up that matters, obviously).
Alternatively you could set up "fresh" trails with same conditions but for a deliberate and controlled screening around what the room temperature typically is. Good luck Thierry ________________________________ From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jan Dohnalek Sent: Monday, November 28, 2011 1:47 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Disappearing crystals We have seen such behaviour connected to temperature fluctuations. Jan On Mon, Nov 28, 2011 at 6:04 PM, Harman, Christine <christine.har...@fda.hhs.gov<mailto:christine.har...@fda.hhs.gov>> wrote: Hi All, I have just noticed a very strange thing and need some help in understanding it. I recently found two crystals in a condition from a screen (0.05M Calcium chloride dihydrate, 0.1M M Bis-Tris pH6.5 and 30% PEG MME 550). The small crystals appeared after a month and started to grow over the next 5 days after I first saw them (see pictures attached). I just check the same drop today and now the crystals are gone. So I was wondering what happened and if anyone experienced this before. Any insight or advice on what to do would be greatly appreciated. Thanks Christine Small 5 days later -- Jan Dohnalek, Ph.D Institute of Macromolecular Chemistry Academy of Sciences of the Czech Republic Heyrovskeho nam. 2 16206 Praha 6 Czech Republic Tel: +420 296 809 390 Fax: +420 296 809 410 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
