When advice on crystallization is needed, it is important to give details
of
the protein concentration, the buffer the protein is in as well as the
method
used to grow the crystals.
Problem: The crystallization conditions are essentially low salt: 100mM
buffer
and only 50mM CaCl2. So the buffer that the protein is in is very
important !!
Fluctuation in the reservoir/drop environment will lead to crystals
dissolving.
Solution: Balance the salt in the reservoir and in the protein:precipitant
drop and make sure
the temperature is kept constant.
Since I do not have all the necessary information, the diagnosis and the
solution proposed
are likely to be wrong!
Enrico.
On Mon, 28 Nov 2011 19:19:49 +0100, YoungJin <yj...@brandeis.edu> wrote:
On 11/28/11 12:04 PM, Harman, Christine wrote:
Hi All,
I have just noticed a very strange thing and need some help in
understanding it. I recently found two crystals in a condition from a
screen (0.05M Calcium chloride dihydrate, 0.1M M Bis-Tris pH6.5 and
30% PEG MME 550). The small crystals appeared after a month and
started to grow over the next 5 days after I first saw them (see
pictures attached). I just check the same drop today and now the
crystals are gone. So I was wondering what happened and if anyone
experienced this before. Any insight or advice on what to do would be
greatly appreciated.
Thanks
Christine
Small 5 days later
Hi Christine,
I had similar experience. In my case, another crystal showed again with
different size a few days later. Sometimes, it seems like it is a common
event to others as well as I heard although my case only takes about a
week to be crystallized. I'd rather wait or just set up again or in a
slightly different way.
Wish you well.
Young-Jin
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
