Hi
Is DNAse essential in your protocol? Maybe it's better to not add DNAse if
you are worried about its presence. If you really need to brake DNA, using
sonication may be enough.
Michel
2007/7/31, Dima Klenchin <[EMAIL PROTECTED]>:
>
> >I have small crystals of protein DNA complex. I am worried
I have small crystals of protein DNA complex. I am worried about the
possibility of presence of some
DNases in the drop (I use DNase in the lysis buffer). I have Tris and
MgCl2 in
the crystallization condition,
which makes me hesitant to use DEPC or EDTA/EGTA respectively. Are there any
other D
Hi all,
I have small crystals of protein DNA complex. I am worried about the
possibility of presence of some
DNases in the drop (I use DNase in the lysis buffer). I have Tris and MgCl2 in
the crystallization condition,
which makes me hesitant to use DEPC or EDTA/EGTA respectively. Are there an
