Hi all, I have small crystals of protein DNA complex. I am worried about the possibility of presence of some DNases in the drop (I use DNase in the lysis buffer). I have Tris and MgCl2 in the crystallization condition, which makes me hesitant to use DEPC or EDTA/EGTA respectively. Are there any other DNase inhibitors that I can use to protect the DNA? Any other precautions one should take while handling DNA Thank you Kumar
Dept. of Biochemistry, Cellular and Molecular Biology, Walters Life Science, # 406, University of Tennessee, TN, Knoxville, USA
