Hi Is DNAse essential in your protocol? Maybe it's better to not add DNAse if you are worried about its presence. If you really need to brake DNA, using sonication may be enough.
Michel 2007/7/31, Dima Klenchin <[EMAIL PROTECTED]>: > > >I have small crystals of protein DNA complex. I am worried about the > >possibility of presence of some > >DNases in the drop (I use DNase in the lysis buffer). I have Tris and > >MgCl2 in > >the crystallization condition, > >which makes me hesitant to use DEPC or EDTA/EGTA respectively. Are there > any > >other DNase inhibitors > >that I can use to protect the DNA? Any other precautions one should take > >while > >handling DNA
