>Date: Wed, 25 Aug 2010 10:19:13 -0400
>From: CCP4 bulletin board (on behalf of Roger Rowlett
>)
>Subject: [ccp4bb] Crystallization of low solubility proteins from
>glycerol-containing solutions
>To: CCP4BB@JISCMAIL.AC.UK
>
> Does anyone have practical expe
Subject: [ccp4bb] Crystallization of low solubility proteins from
glycerol-containing solutions
Does anyone have practical experience crystallizing low solubility proteins
from solutions containing significant (10-20%) glycerol?
Dear Roger,
Since you already have initial crystallisation conditions and crystals you could
try dialysis buttons to further reduce the glycerol content (and hence get
bigger crystals). They are available e.g. from Hampton, the minimum volume is 5
mul and I find they require a little skill to set
Hi Roger,
I crystallized a protein that started in solution with 250 mM NaCl, 50%
glycerol, and 50 mM Arg+Glu. I initially used traditional microbatch under
paraffin oil to get crystallization hits (where protein and screen are 1:1;
glycerol ends up at 25%). Then, I found that setting up sittin
Does anyone have practical experience
crystallizing low solubility proteins from solutions containing
significant (10-20%) glycerol? We can get small crystals by mixing
4:1 ratios of protein to well solution, but the drops do not
concentrate back to the well solu
