Hi Roger, I crystallized a protein that started in solution with 250 mM NaCl, 50% glycerol, and 50 mM Arg+Glu. I initially used traditional microbatch under paraffin oil to get crystallization hits (where protein and screen are 1:1; glycerol ends up at 25%). Then, I found that setting up sitting drops like a microbatch worked even better (where protein and screen are 1:1 and the well solution is the protein buffer and screen also 1:1).
Best thing about it was the crystals were already cryoprotected and ready to put on the beam. See Del Campo & Lambowitz Acta Cryst. (2009). F65, 832–835. If you have any questions let me know. Best, Mark Research Associate Lambowitz Laboratory UT Austin
