Have you tried to use glycerol or ethylene glycol as the precipitant? What happens when you go to 50% or higher concentrations?
_____ From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Roger Rowlett Sent: Wednesday, August 25, 2010 9:19 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Crystallization of low solubility proteins from glycerol-containing solutions Does anyone have practical experience crystallizing low solubility proteins from solutions containing significant (10-20%) glycerol?
