Dear Fritz,
You are right. The recollection of that striking 2015 paper was somehow
too strong to be 100% faithful: these are two different modes of using the
ligand deposited on a solid support by drying a solution of it, to avoid the
DMSO problem.
Best wishes,
Gerard.
--
On
:)
Gerard,
"soaking"!
Thanks for the reference!
cheers
guenter
Dear Guenter,
The first description of the idea behind this technique that I am aware
of is that published almost 10 years ago by a French group under the name of
"'dry' co-crystallisation" in the following paper:
Dear Guenter,
The first description of the idea behind this technique that I am aware
of is that published almost 10 years ago by a French group under the name of
"'dry' co-crystallisation" in the following paper:
http://dx.doi.org/10.1107/S1399004715010342
Best wishe
Hi Amit,
like Artem wrote below, even despite poor solubility, soaks with dried
compounds work well (also in our hands).
We adapted it from the work of T. Barthel, J. Wollenhaupt and Manfred
Weiss. Also colleagues from industry report good results.
We add compound dissolved in DMSO to the crysta
Wednesday, October 2, 2024 at 2:51 PM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
mailto:CCP4BB@JISCMAIL.AC.UK>>
Subject: [ccp4bb] Co-Crystallization with drug molecule
Hi everyone,
I am trying to crystallize a protein with a drug molecule. The protein
concentration
*Subject: *[ccp4bb] Co-Crystallization with drug molecule
Hi everyone,
I am trying to crystallize a protein with a drug molecule. The protein
concentration is 15.5 mg/ml, the drug stock concentration is 10 mM,
and the drug is dissolved in DMSO. I am adding the drug to a final
concentration of
ubject: [ccp4bb] Co-Crystallization with drug molecule
Hi everyone,
I am trying to crystallize a protein with a drug molecule. The protein
concentration is 15.5 mg/ml, the drug stock concentration is 10 mM, and the
drug is dissolved in DMSO. I am adding the drug to a final concentration of 1
mM in
, delete the e-mail and do not disclose its contents to
any person. Any unauthorized review, use, disclosure, copying or distribution
is strictly prohibited.
Von: CCP4 bulletin board Im Auftrag von Tom Peat
Gesendet: Mittwoch, 2. Oktober 2024 23:23
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] Co-
K
Subject: Re: [ccp4bb] Co-Crystallization with drug molecule
Dear Amit
As David already pointed out, all proteins are different and it's hard to say
in advance what amount of DMSO may work (or not).
An additional concern is that DMSO can also interfere with ligand binding
(cases from m
Dear Amit
As David already pointed out, all proteins are different and it's hard to
say in advance what amount of DMSO may work (or not).
An additional concern is that DMSO can also interfere with ligand binding
(cases from my personal past history), especially if these
inhibitors/ligands are on
on behalf of amit gaur
Sent: Wednesday, October 2, 2024 2:51 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Co-Crystallization with drug molecule
Hi everyone,
I am trying to crystallize a protein with a drug molecule. The protein
concentration is 15.5 mg/ml, the drug stock concentration is
Hi everyone,
I am trying to crystallize a protein with a drug molecule. The protein
concentration is 15.5 mg/ml, the drug stock concentration is 10 mM, and the
drug is dissolved in DMSO. I am adding the drug to a final concentration of
1 mM in 100 ul of protein, and the DMSO volume is 10 ul for
Co
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