Dear Adriana,
this depends on the enzyme i.e. does your enzyme hydrolyse the
alpha-beta or beta-gamma bond?
If it hydrolyses the beta-gamma bond, options mentioned are indeed:
-AMPPNP (Adenylyl-imidodiphosphate)
-AMPPCP(β,γ-Methyleneadenosine 5′-triphosphate)*
*-AMPPP(S) (ATP-γ-S)
-ADP-BeFx
-
Hi,
one word of caution with Metal fluoride complexes. Though they can
provide valuable inside in the reaction mechanism, even better than some
ATP-analogues, one has to be careful what metal to use to get the right
complex. See review below, which summarises the problems which can
occur, es
Thanks every one for the great help. I am loaded with all the useful
information.
best
Adriana
On Tue, May 2, 2017 at 9:07 PM, Deaconescu, Alexandra <
alexandra_deacone...@brown.edu> wrote:
> Hi,
>
> ATPgS is an option, but it is slowly hydrolysable, so depending on your
> enzyme, it might not
Hi,
ATPgS is an option, but it is slowly hydrolysable, so depending on your
enzyme, it might not be the best one to use.
Cheers,
Alexandra
On Tue, May 2, 2017 at 2:56 PM, Aaron Thompson
wrote:
> ATP-gamma-S is another option. There are 123 public structures
> containing this ligand (ligand I
ATP-gamma-S is another option. There are 123 public structures containing
this ligand (ligand ID AGS).
Cheers,
Aaron
On Tue, May 2, 2017 at 11:42 AM, Kevin Jude wrote:
> ADP-BeFx and ADP-AlFx (the latter mimics a dissociative transition state).
> The fluoride compounds can be made by mixing
ADP-BeFx and ADP-AlFx (the latter mimics a dissociative transition state).
The fluoride compounds can be made by mixing BeCl2 or AlCl3 with excess NaF
and then used at say a 3:1 excess over ADP. Be sure to dissolve the
chloride compounds in a fume hood!
Also ADP-VO4, which can be prepared as descr
Dear Adriana,
-AMPPNP (Adenylyl-imidodiphosphate) or
-AMPPCP (β,γ-Methyleneadenosine 5′-triphosphate)
You can be buy them easily.
Best - Gert
Philipps University Marburg
LOEWE Center for Synthetic Microbiology &
Dep. of Chemistry
Dr. Gert Bange
Han
Hi
What are good ATP non hydrolyzable analog for crystallization?
best
Adriana
