Dear Adriana,
this depends on the enzyme i.e. does your enzyme hydrolyse the
alpha-beta or beta-gamma bond?
If it hydrolyses the beta-gamma bond, options mentioned are indeed:
-AMPPNP (Adenylyl-imidodiphosphate)
-AMPPCP(β,γ-Methyleneadenosine 5′-triphosphate)*
*-AMPPP(S) (ATP-γ-S)
-ADP-BeFx
-ADP-AlFx
Beware though, by replacing the bridging beta-gamma oxygen in ATP to N
(NH; AMPPNP) or C (CH2; AMPPCP) you loose (O to CH2) the possibility of
forming or you reverse (O to NH; from acceptor to donor) a hydrogen bond
here. Thus, the use of the latter two compounds can have adverse effects
(and you do not want to get into an argument with a referee in the
future). ATP-γ-S is probably the best option if, bound the the protein,
hydrolysis is slow enough or the complex crystallizes fast enough (cold
room!).
ADP-BeFx and ADP-AlFx could be options but again, the charge
distribution is not equivalent to ATP and it requires additional amino
acid side chains that coordinate the
AlFx or BeFx group.
Good luck,
Jeroen
Am 02.05.17 um 18:04 schrieb Adriana Sene:
Hi
What are good ATP non hydrolyzable analog for crystallization?
best
Adriana
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It is invariably the case that high resolution X-ray structures show
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