Hi, ATPgS is an option, but it is slowly hydrolysable, so depending on your enzyme, it might not be the best one to use.
Cheers, Alexandra On Tue, May 2, 2017 at 2:56 PM, Aaron Thompson <aaron.a.thomp...@gmail.com> wrote: > ATP-gamma-S is another option. There are 123 public structures > containing this ligand (ligand ID AGS). > > > Cheers, > > > Aaron > > On Tue, May 2, 2017 at 11:42 AM, Kevin Jude <kj...@stanford.edu> wrote: > >> ADP-BeFx and ADP-AlFx (the latter mimics a dissociative transition >> state). The fluoride compounds can be made by mixing BeCl2 or AlCl3 with >> excess NaF and then used at say a 3:1 excess over ADP. Be sure to dissolve >> the chloride compounds in a fume hood! >> >> Also ADP-VO4, which can be prepared as described in Goodno CC. Myosin >> active-site trapping with vanadate ion. Meth Enzymol. 1982;85 Pt B:116–23. >> >> Best wishes >> Kevin >> >> On Tue, May 2, 2017 at 9:04 AM, Adriana Sene <adrianasen...@gmail.com> >> wrote: >> >>> Hi >>> What are good ATP non hydrolyzable analog for crystallization? >>> >>> best >>> >>> Adriana >>> >> >> >> >> -- >> Kevin Jude, PhD >> Research Specialist, Garcia Lab >> Departments of Molecular & Cellular Physiology and Structural Biology >> Stanford University School of Medicine >> Beckman B177, 279 Campus Drive, Stanford CA 94305 >> Phone: (650) 723-6431 >> > >
