BS”D
Dear Garib,
This is a deposited structure, not something I did.
They used REFMAC 5.8.0107
Harry
On 15 Oct 2018, at 12:25 PM, Garib Murshudov
mailto:ga...@mrc-lmb.cam.ac.uk>> wrote:
Dear Harry,
Could you please send the script or log file from refmac. It should have not
happened, bu
BS”D
Dear All,
I would just like to know how refmac handles the following setup.
A deposited structure (2.8Å resolution) is a homotrimer protein (chains A, B,C)
with bound dsDNA (chains D, E), and some water molecules.
There is NCS (type: local) defined between the three protein chains (A t
UCLA anisotropy server. Using the scaled mtz should solve
your problem.
Best,
Herman
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Appu
kumar
Gesendet: Donnerstag, 2. Juli 2015 05:17
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] ANISOU in pdb and density improvement
Hello All,
Sorry for giving incomplete information. The protein is 75KDa membrane
protein and it exists as tetramer in ASU. Structure is solved by MR.
Overall completeness of data is 98% wiith multiplicity of 4.8. Density
looks great after refinement having ANISOU record in PDB.
Any suggestions an
4 Angstrom resolution is pretty low and there has to be other info
associated with that to get more help from here.
How big is your protein? How are you solving the phases? How complete is
your data at that resolution? What kind of multiplicity are you getting? I
think you have other issues that ar
Dear CCP4 users,
I am refining a structure at 4A resolution. Crystal diffracted
anisotropically and after refinement in both PHENIX and REFMAC, electron
density in one of the domain of protein is represented by discontinuity and
poor maps. Therefore i did the aniosotropy correction using anisotropy
Dear friends,
if the data is of high enough resolution, wouldn't be more reasonable to
attempt anisotropic refinement (constrained with TLS or refining
individual anisotropic ADP), or mixed one - some atoms are isotropic and
some anisotropic, rather than struggle with file conversions and gett
Dear Tim Fenn,
That's better : )
On Mon, 16 Mar 2009 11:01:34 +0800
Sheng Li wrote:
>
> Please read the coordinate file with alwyn's O, and then save it to
> another file. The ANISOU lines will be removed.
>
only if you use s_a_i - pdb_read will preserve ANISOU.
It might be easier to just gr
On Mon, 16 Mar 2009 11:01:34 +0800
Sheng Li wrote:
>
> Please read the coordinate file with alwyn's O, and then save it to
> another file. The ANISOU lines will be removed.
>
only if you use s_a_i - pdb_read will preserve ANISOU.
It might be easier to just grep them out:
grep -v "^ANISOU" fo
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=== 2009-03-16 10:48 riya doreen 您在来信中写到: [ccp4bb] ANISOU===
Hello Everyone,
I am trying to refine a structure with CNS. The initial
Hello Everyone,
I am trying to refine a structure with CNS. The initial model consists of
protein with ANISOU B factors. However, when I run generate, the program
crashes with the message
%COOR-ERR: unrecognized command:
ANISOU
^^
I want to keep the aniso B factors since the resolution of my
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