Dear CCP4 users, I am refining a structure at 4A resolution. Crystal diffracted anisotropically and after refinement in both PHENIX and REFMAC, electron density in one of the domain of protein is represented by discontinuity and poor maps. Therefore i did the aniosotropy correction using anisotropy server. After anisotropy correction using anisotropy server, the electron density becomes resonable for 4A map as evident by appearance of electron density around some bulky sidechains and discontinuity in electron density is diminished. When i look into the pdb, I found that pdb contain ANISOU records for each atoms.
I tried same things with other data sets and same data set again (data set described above) of same protein. After refinement, the ANISOU records are not present for each atoms in refined pdb and the electron density is discontinuous and poor. I am wondering why one of the anisotropically corrected MTZ by diffraction server gives the ANISOU records with the good density after refinement and other mtz file do not produce ANISOU record in pdb and poor density map. I would appreciate your help regarding this issue. Thank you Appu
