Hello All, Sorry for giving incomplete information. The protein is 75KDa membrane protein and it exists as tetramer in ASU. Structure is solved by MR. Overall completeness of data is 98% wiith multiplicity of 4.8. Density looks great after refinement having ANISOU record in PDB.
Any suggestions and guidance will be much appreciated Thanks you Appu On 1 July 2015 at 21:14, xaravich ivan <xaravich.i...@gmail.com> wrote: > 4 Angstrom resolution is pretty low and there has to be other info > associated with that to get more help from here. > How big is your protein? How are you solving the phases? How complete is > your data at that resolution? What kind of multiplicity are you getting? I > think you have other issues that are much more pressing than what you are > asking here!!! > As you are able to get 4A data it is evident that you can crystallize your > protein. Congrats!!!! Now, concentrate on that and try to get higher > resolution data from better diffracting crystals and it might be a much > easier experience, (unless you trying to solve a huge ribosomal complex > kind of protein and in that case 4 angs is amazing!!!!) > cheers > > Ivan > > On Wed, Jul 1, 2015 at 5:57 PM, Appu kumar <appu.kum...@gmail.com> wrote: > >> Dear CCP4 users, >> I am refining a structure at 4A resolution. Crystal diffracted >> anisotropically and after refinement in both PHENIX and REFMAC, electron >> density in one of the domain of protein is represented by discontinuity and >> poor maps. Therefore i did the aniosotropy correction using anisotropy >> server. After anisotropy correction using anisotropy server, the electron >> density becomes resonable for 4A map as evident by appearance of electron >> density around some bulky sidechains and discontinuity in electron density >> is diminished. When i look into the pdb, I found that pdb contain ANISOU >> records for each atoms. >> >> I tried same things with other data sets and same data set again (data >> set described above) of same protein. After refinement, the ANISOU records >> are not present for each atoms in refined pdb and the electron density is >> discontinuous and poor. >> >> I am wondering why one of the anisotropically corrected MTZ by >> diffraction server gives the ANISOU records with the good density after >> refinement and other mtz file do not produce ANISOU record in pdb and poor >> density map. >> >> I would appreciate your help regarding this issue. >> Thank you >> Appu >> > >
