[ccp4bb] Crystal growing in one direction...need suggestions

Dear all, I have crystallized a protein in MPD which is thin and growing in one direction only. I have tried ethonal, DMSO, Proline, Glucose, Urea, Sucrose, Detergent kit from Hampton and lot more alongwith temperature variation. Need suggestions regarding improvement of xtal quality, THICKNESS. Th

[ccp4bb] To improve xtal quality

Dear all, I am trying to improve the quality of a xtal, crystallizing with MPD as a precipitant at 16 degree C with thin plate like xtals. I would appreciate suggestions to have diffraction quality xtals (thick). I am using proline, glycine, glucose, sucrose as an additive.Thanx in advance. shives

[ccp4bb] sumary of "Avoiding protein from going to inclusion body..."

don, WC1E 7HX. - Peter J. Miller wrote On 2 Apr 2008, at 12:19, Brenda Patterson wrote: Lower temperature, use chaperones (e.g. TAKARA set), refolding? Quoting shivesh kumar <[EMAIL PROTECTED]>: Dear all, Sorry for the off-topic question... What can be done to avoid a protein going inside i

[ccp4bb] "inclusion body"

Dear all, Sorry for the off-topic question... What can be done to avoid a protein going inside inclusion body.The gene is cloned in pET30a with C-ter his tag and expressed in BL21-DE3 from 37 to 18C for 3-4 hr with .5mM of IPTG,it is going to inclusion body.All suggestions are welcome. Thanx in ad

[ccp4bb] libstdc++.so.5

Dear all, We have installed CCP4 6.0.2 in Fedora core 8. While running the program we are getting the following error message- error while loading shared lilbraries libstdc++.so.5, cannot open shared object file: no such file or directory. Please suggest how to get this library file... Thanx in ad

[ccp4bb] cryoprotectant for crystals in isopropanol

it with higher PEG's also like 1K, 2K, 4K.Lets C.Since the condition is having isopropanol also and it is reported that mounting with isoprop is very difficult and it evaporates as soon as we open the slides. Thanx in advance... Shivesh Kumar

[ccp4bb] isopropanol as a precipitant

Dear all Sorry for non-CCP4 query. I have crystallized a 7kDa protein in 50-60% of isopropanol,pH 4.0-4.6.Theinteresting thing is that the xtal appears within 5 hrs at 16 degree. The protein conc is 5mg/ml and drop size is 3+1 with mother liquor 300 micro lt.Numerous xtals appears and but small is

[ccp4bb] Twinned crystals to single crystal

Dear all, I have crystallized a protein in 63% of MPD at pH 3.8-4.6.The crystals are twinned but with sharp edges.I welcome all the suggestions to get single crystals.Also,I have needles of other mutant protein,the problem is during concentration,it gets precipitated even in the centricon.What shou

[ccp4bb] off-topic-Heat shock protein gets precipitated

precipitation.Also appreciate worth suggestions from groups working with chaperonins.Thanx in advance. Shivesh kumar

Re: [ccp4bb] crystal with precipitation

. Shivesh kumar

[ccp4bb] crystal with precipitation

additive with the mother liquor to avoid precipitation.But,the precipitation is still there.The volume of the mother liquor is 500 microlt.The drop size is 2+2.I welcome all the suggestions regarding avoiding the precipitation.Thanx is advance. Shivesh kumar

[ccp4bb] needles vs crystals

I do to get good crystals,the temp. I am trying is 16C.Thanx in advance for the suggestions. Shivesh Kumar

[ccp4bb] process SeMet labelled data

Dear all, I have a data set at 2.2A, of the selenomethionene labelled protein.Howshould I process the data.Thanx for the help. Shivesh

[ccp4bb] molrep

Dear all I am trying MR in molrep(CCP4) with P21212.mtz.I have tried nearly all space group.Rfact and corr.coeff is 57-59% and 23-25% resp.what should I try to lower the Rfact.The data is at 2.2A.Any suggestions are welcome.Thanx in advance. Shivesh

[ccp4bb] to detwin the twinned data

Dear all, Can u suggest me any method to detwin the twinning data(2.2A).Any suggestions are welcome.Thanx in advance. Shivesh

[ccp4bb] DPC-peptide crystal

Dear all I am trying to crystallize one DPC bound small peptide.Small cystals are appearing.How can I grow it bigger for mounting.Any suggestions are welcome.Thanx in advance. Shivesh

[ccp4bb] peptide crystal

Dear all I am trying one DPC bound peptide.Can anyone suggest me which precipitant and additives I should add.Thanx in advance. Shivesh

[ccp4bb] crystal

Dear all Sorry for unrelated query.I have crystallized a protein which is at 40% of MPD.But the problem is that I get several crystals but these are small.Thecrystals diffract upto 3A.I welcome any suggestion to make the crystals big enough to diffract at high resolution.What additives I can add??

[ccp4bb] peptide crystallization

Dear all, Can u suggest me about the peptide crystallization,some references.Should I try it with PEG or MPD.It is 10-15 residues long.Thanx in advance. S...