[ccp4bb] isopropanol as a precipitant

[shivesh kumar]

Dear all
Sorry for non-CCP4 query.
I have crystallized a 7kDa protein in 50-60% of isopropanol,pH
4.0-4.6.Theinteresting thing is that the xtal appears within 5 hrs at
16 degree.
The protein conc is 5mg/ml and drop size is 3+1 with mother liquor 300 micro
lt.Numerous xtals appears and but small is size.Do anyone can share their
experience with Isopropanol as a precipitant and to improve the xtal quality
with other precipitants.All suggestions are welcome.
Thanx in advance.
Shivesh