[ccp4bb] Twinned crystals to single crystal

[shivesh kumar]

Dear all,
I have crystallized a protein in 63% of MPD at pH 3.8-4.6.The crystals are
twinned but with sharp edges.I welcome all the suggestions to get single
crystals.Also,I have needles of other mutant protein,the problem is during
concentration,it gets precipitated even in the centricon.What should I do to
avoid this precipitation to have a concentrated protein.Thanx in advance for
the suggestions.
Shivesh