Re: [ccp4bb] Interesting pattern on a crystallization drop

2019-03-28 Thread jai mohan
May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his work on Dancing Droplets, briefing the surface tension and evaporation ^ the rule of two component fluids. # Since your precipitant contain PVP a shape controlling agent #https://med.stanford.edu/news/all-news/2015/03

Re: [ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :(

2018-08-15 Thread jai mohan
Dear Careina, Please make sure that your crystals are protein or salt, possibly using Izit dye.Note: there is no correlation so far hypothesized on the diffraction of ugly / beautiful / needle / plate type crystalsI believe! With best regardsS.M.Jaimohan PhD On Tuesday, 14 August, 2018,

[ccp4bb] Separating Monomers and Dimers

2017-06-27 Thread jai mohan
Dear all,I am working on a Red FluorescentProtein (His-Tag) molecular weight around 27kDa. After purification I ran a SDSpage, the band at 27kDa confirms the monomer. The protein was stored at -20C, aweek later again I ran a gel, this time I saw another new band between 50-60kDa, it confirmsthe

Re: [ccp4bb] Crystal morphology

2015-05-17 Thread jai mohan
Dear Bishwa,What about Hamptons Izit dye. S.M.Jaimohan, Ph.D On Monday, 18 May 2015 7:50 AM, Engin Özkan wrote: Magnesium phosphate crystals? I've definitely seen salt crystals with that morphology before. Check out the Ksp for Mg3(PO4)2. Engin On 5/17/15 6:45 PM, Bishwa Subedi

Re: [ccp4bb] Offtopic: Software to closely visualize interacting partnets in protein complex

2015-03-10 Thread jai mohan
Dear Debasish,For GUI try Pymol, Chimera, CCP4MG.Also try CCP4-CONTACT not an GUI, but you get all possible interactions between two chains.best wishes  S.M.Jaimohan, Ph.D On Tuesday, 10 March 2015 3:58 PM, Debasish Kumar Ghosh wrote: Dear All, Apologies for this little off-topic

Re: [ccp4bb] Incubator for crystallization

2014-11-11 Thread jai mohan
Dear Ulrike, Molecular dimensions incubator model MD5-601 works pretty good in our lab. -Peltier type -low vibration -fully programmable up to 99 days -temperature up to 4C -accommodate low space -with RS232 Check the following links | From archives related to your question posted previously.

Re: [ccp4bb] software for powder pattern

2014-08-20 Thread jai mohan
Hi, Rigaku's PDXL software might fullfill your requirement, but I don't think its academic free. You may also try google the PDXL patch programs ICSD database | EXPO2009. Best wishes, Dr. S.M. Jaimohan On Wednesday, 20 August 2014 8:06 AM, Nancy Naguib wrote: Dear All i would like your advi

[ccp4bb] EDS server - R-value

2014-04-04 Thread jai mohan
Dear all, Sorry for the off-topic Question. I just tried to extract files *.* from EDS server for a PDB entry. The page tells us that There is no map available for this entry (), because our automatic script failed to produce an electron density map with an R-value (0.309) within 5 percentage

Re: [ccp4bb] size of a flexible pdb structure

2014-03-05 Thread jai mohan
Dear Rajan, Since you raised your question about calculating the radius of your molecule. here my suggestion, a bit long back I heard about the program HYDROPRO I am not sure its going to be work for you, but you may give a try. I think the program calculates the radius of gyration !  best wishes,

Re: [ccp4bb] high Rwork / Rfree after MR

2014-02-04 Thread jai mohan
Dear Shanti Pal Gangwar, You are trying to solve your crystal structure of what! whether its a peptide or protein or enzyme or nucleic acid? There are several reasons behind getting a high Rmerge, it not only depends on the data quality. What are all the efforts! that you have applied to solved

Re: [ccp4bb] 'ERROR' in merging two data sets

2010-09-30 Thread jai mohan
ge the two sets of measurements into a single data set? Eleanor jai mohan wrote: > > Dear all crystallographers, Recently I have collected two data sets from two > different crystals, first data at 2.6A and second is at 1.9A, the datasets > were isomorphous. I tried to  merg

[ccp4bb] 'ERROR' in merging two data sets

2010-09-30 Thread jai mohan
Dear all crystallographers, Recently I have collected two data sets from two different crystals, first data at 2.6A and second is at 1.9A, the datasets were isomorphous. I tried to  merge the two dataset as a single MTZ file using CAD (CCP4i), but it is unsucces

[ccp4bb]

2009-10-24 Thread jai mohan
Dear Askok, There is many programs & online servers to do this task, i prefer you profit server, http://www.bioinf.org.uk/profit/    best regards jaimohan     --- On Sat, 24/10/09, ashok kumar wrote: From: ashok kumar Subject: [ccp4bb] To: CCP4BB@JISCMAIL.AC.UK Date: Saturday, 24 October, 200

Re: [ccp4bb] Regarding BMCD

2009-06-12 Thread jai mohan
Dear Nishant, You may follw this link below, after you indicate the protein/enzyme/nucleic acid name don't hit ENTER [probably it wont work] CLICK submit. http://xpdb.nist.gov:8060/BMCD4/index.faces;jsessionid=b196fdf1b356c8293b85a9f3981d   jaimohan --- On Fri, 12/6/09, Nishant Varshney wrote: