ral Biology Laboratory
> SLS, JNU,
> New Delhi-110067
>
--
Vandana kukshal
t;
>
>
>
>
> --
> patr...@douglas.co.ukDouglas Instruments Ltd.
> Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK
> Directors: Peter Baldock, Patrick Shaw Stewart
>
> http://www.douglas.co.uk
> Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034
> Regd. England 2177994, VAT Reg. GB 480 7371 36
>
>
--
Vandana kukshal
reenshot?
>
> I am looking forward to getting your reply.
>
> Cheers,
>
> Dialing
>
--
Vandana kukshal
troduce me a server so that I can convert it to another PDB file starting
> from 200 to 300?
>
> Cheers,
>
> Dialing
>
--
Vandana kukshal
n?
>
> ** **
>
> Thanks in advance****
>
> ** **
>
> Amit
>
--
Vandana kukshal
CP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] How can improve diffraction quality
>
> Dear ccp4 user
>
> I am facing one crucial problem regarding diffraction. Actually the size of
> my crystal is good enough 0.5mm but it was diffracted only 4 A.
>
> The conditions of crystallization recipes are 4.5% PEG 1000, 0.1M Tris and
> 25mM Na citrate. I really need your suggestions regarding how can i
> improve my diffraction quality?
>
> Your support is highly appreciable.
>
> Best Regards
>
> AFSHAN
>
--
Vandana kukshal
.7% -15% 0.435 119774
>
> Note that I/SIGMA of each resolution shell is <2.5, so how should I do to
> process the dataset properly? Any suggestion about this super ice rings?
> Thanks!
>
> Tiantian
>
> --
> Shanghai Institute of Materia Medica, Chinese Academy of Sciences
> Address: Room 101, 646 Songtao Road, Zhangjiang Hi-Tech Park,
> Shanghai, 201203
>
--
Vandana kukshal
chain
atom is this wil be correct way to do these calculation. welcome ur
valuable sugessions .
--
Vandana Kukshal
Postdoc Fellow
structure biology group
international Center For Genetic Engineering and Biotechnolgy
India
t;
> --
> Konstantin Korotkov, Ph.D.
>
> Research Scientist
> University of Washington
> Department of Biochemistry
> Box 357742
> Seattle, WA 98195-7742
>
> (206)616-4512
> k...@u.washington.edu
> --
--
Vandana Kukshal
Postdoc Fellow
structure biology group
international Center For Genetic Engineering and Biotechnolgy
India
molecules for a given crystal structure. But I'm looking for some
> program/software (for batch) by which I can find out the number
> of symmetry related molecules (distance cutoff <= 5A) interacting with
> a given chain in a crystal structure.
>
> Thanking you,
> suku
>
t;> The solution is getting clear in the room temperature within 5 mins when I
>> set up the trays.
>>
>> I would like to take any advice. I would appreciate your input.
>>
>> Best
>>
>> Min
>>
>
>
--
Vandana Kukshal
Postdoc Fellow
structure biology group
international Center For Genetic Engineering and Biotechnolgy
India
mit Luthra, Ph.D.
> Post-Doctoral Fellow
> The Radolf Laboratory
> Department of Medicine
> University of Connecticut Health Center
> [w] http://spirocheteresearch.uchc.edu
>
--
Vandana Kukshal
Postdoc Fellow
structure biology group
international Center For Genetic Engineering and Biotechnolgy
India
better shape, did I got the point?
>
> Best wishes
>
> Xinghua Qin
>
> --
> Xinghua Qin
> Room 4022, Center for Life Sciences,
> China Agricultural University,
> No.2, Yuan Ming Yuan West Road, Haidian District，
> Beijing，China，100193
> Tel: +86-10-62732672
>
>
hello
if u are getting merged spot then try to increase detector distance it may
work . instead of this u try to reduce delta phi (gonimeter phi angle per
frame) default it is 1 degree u can try 0.5, 0.25 degree.
I take it to synchrotron which is far away?
>
> thanks
> Careina
>
>
--
Vandana Kukshal
Postdoc Fellow
structure biology group
international Center For Genetic Engineering and Biotechnolgy
Aruna Asaf Ali Marg
110 067 New Delhi
INDIA
> > to have an idea of the intrinsically disordered regions in the protein,
>> transmemebrane regions and disulfide bonds that would certainly help you in
>> initiating in the right direction.
>> >
>> > Best wishes
>> > Gauri
>> >
>>
d TCEP in ur buffer instead
of Beta mercaptoethanol.
for one of my crystallization experiment i used 1- 10 mM of Beta
mercaptoethanol.
--
vandana kukshal
CSIR-senior research fellow
X ray lab ,MSB division
central drug research institute
lucknow
--
Vandana Kukshal
Postdoc Fellow
structure b
hello sir ,
recently i have collected one data of 3.0 A of a
protein having no sequence homology with any known PDB .
but
while fold prediction i got 100 % identical fold with some of the
protein .
space group of my protein is P622 and showing 6 molecule
in a assymetric unit.
the homologous
how to make covalent
bond between ligand and protein residue by using CCp4 package
i have one Query .
after using phenix auto build for building model R factor and R free
reduced but B factor is increesed for all the atom .what next i should do
to decrease the B factor of atoms.
hello
this is not a new question i was searching this in archives but i
did'nt get .
just send me link for this .
i want to link AMP covalently with lysine in my structure (phosphoamide
bond)how i will do this in coot and refmac .
in turbo there is option make bond but in this n
i have one query. that if we have a multi domain protein structure to
solve ,and we know about only one small domain of the protein for MR .
other domain structure is not known then how we can proceed.
is there any procedure to utilize phase from the solution with known
domain .
hello
i have 3.25 A data of multidomain protein with 4 individual domain
.one domains structure is already known . and for others domain 40 %
simmilar structure is known . when i am running phaser with one known
domain i am getting the solution but after getting solution i am
you can try glutathione and cysteine as reducing agent with conc
0.01-0.001M or higher
total number of atoms are 5113
i am using restrain refmac for refine ment and coot for fiting
its resolution range is 116.248 -3.00
number of used reflections 20215
the R factor is 27.7
and R free is 27.0
and if i am doing further restrain refinement with 0.03 geometry weight
R factor is going down to 25.5
but R f
hi
i am solving one structure
in which after refinement i got R factor=27
and R free =27.7
i want to know that is it correct or i did some over refinment
the Resolution is 3.0 A
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